WB result of Collagen XVII Recombinant Rabbit mAb
Primary antibody: Collagen XVII Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HaCaT whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 150 kDa
Observed MW: 130 kDa
This blot was developed with high sensitivity substrate
Collagen XVII Recombinant Rabbit mAb (S-1636-5)
Collagen XVII Recombinant Rabbit mAb (S-1636-5)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Collagen XVII |
| Synonyms | Collagen alpha-1(XVII) chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; COL17A1; BP180; BPAG2 |
| Immunogen | Synthetic Peptide |
| Location | Membrane, Cell junction |
| Accession | Q9UMD9 |
| Clone Number | S-1636-5 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, HaCaT |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
| ICC | 1:500 | Hu |
Background
Collagen XVII, also known as COL17 or BP180, is a transmembrane protein that plays a crucial role in maintaining the adhesion between the epidermis and the basement membrane in the skin. It is a component of hemidesmosomes, which are multiprotein complexes that mediate the adhesion of keratinocytes to the underlying membrane. It is involved in cell-cell adhesion and signaling, particularly in immune cell transmigration and angiogenesis. The extracellular domain of COL17 can be physiologically cleaved from the cell surface by ADAM family proteins in a process known as ectodomain shedding, which is thought to be associated with the migration and proliferation of keratinocytes. The C-terminal cleavage of COL17 may be associated with basement membrane formation. Collagen XVII is involved in skin homeostasis, aging, and wound repair. It mediates the interactions of stem cells with surrounding cells and the matrix to regulate these processes. Changes in COL17 expression and function are associated with skin aging, as it affects the balance of symmetric and asymmetric cell divisions in epidermal stem cells. COL17 has also been implicated in skin cancers, such as squamous cell carcinoma, basal cell carcinoma, and malignant melanocytic tumors.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human skin. Anti-Collagen XVII antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human esophagus. Anti-Collagen XVII antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Collagen XVII antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Collagen XVII antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human transitional cell cancer. Anti-Collagen XVII antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in A431 cells (top panel) and negative staining in MCF7 cells (below panel). Anti-Collagen XVII antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
