IHC shows positive staining in paraffin-embedded rat cartilage. Anti-Collagen II antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Collagen ll Recombinant Rabbit mAb (S-3360)
Collagen ll Recombinant Rabbit mAb (S-3360)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Collagen II |
| Synonyms | Collagen alpha-1(II) chain; Alpha-1 type II collagen; COL2A1 |
| Location | Secreted |
| Accession | P02458 |
| Clone Number | S-3360 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:200 | Hu, Rt |
Background
Collagen II, also known as type II collagen, is a crucial fibrillar protein predominantly found in cartilaginous tissues, including hyaline cartilage and articular cartilage, where it constitutes up to 90% of the collagen content. It is synthesized by chondrocytes and is essential for maintaining the structural integrity and resilience of cartilage. Collagen II is formed by homotrimers of the collagen type II alpha-1 chain (α1(II)), and its molecular structure includes a central triple-helical domain flanked by N- and C-propeptides. This collagen is also present in the vitreous humor of the eye, intervertebral discs, and the inner ear. Mutations in the gene encoding Collagen II (COL2A1) are associated with various skeletal and connective tissue disorders, such as Stickler syndrome and Kniest dysplasia.
Picture
Picture
Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded rat cartilage. Anti-Collagen II antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
