IHC shows positive staining in paraffin-embedded human colon. Anti-Collagen III antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Collagen III Recombinant Mouse mAb (S-3838)
Collagen III Recombinant Mouse mAb (S-3838)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Collagen III |
| Synonyms | Collagen alpha-1(III) chain; COL3A1 |
| Location | Secreted |
| Accession | P02461 |
| Clone Number | S-3838 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
Collagen III is a homotrimeric fibrillar protein composed of three identical α1(III) chains wound into a triple helix, encoded by the COL3A1 gene, that copolymerizes with collagen I to endow skin, blood vessels and hollow organs with extensibility and compliance, and whose mutations or deficiency cause the life-threatening vascular fragility syndrome Ehlers–Danlos syndrome type IV.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Collagen III antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Collagen III antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Collagen III antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Collagen III antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Collagen III antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skin. Anti-Collagen III antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
