WB result of CNBP Recombinant Rabbit mAb
Primary antibody: CNBP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: K-562 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDa
CNBP Recombinant Rabbit mAb (S-4288-3)
CNBP Recombinant Rabbit mAb (S-4288-3)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CNBP |
| Synonyms | CCHC-type zinc finger nucleic acid binding protein; Cellular nucleic acid-binding protein (CNBP); Zinc finger protein 9; RNF163; ZNF9; CNBP |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Endoplasmic reticulum |
| Accession | P62633 |
| Clone Number | S-4288-3 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, HepG2, K-562, Jurkat, LNCaP, Neuro-2a, mouse brain, PC-12, rat brain, COS7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
| IP | 1:50 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
CNBP (Cellular Nucleic Acid-Binding Protein), also known as ZNF9, is a highly conserved, single-stranded nucleic acid-binding protein that functions as a versatile nucleic acid chaperone. It plays a critical role in regulating both transcription and translation by remodeling nucleic acid secondary structures and modulating the activity of trans-acting factors. CNBP is essential for maintaining sustained immune responses (such as prolonged IL-6 expression) and proper embryonic development. Clinically, abnormal expansions of the CCTG repeat in the first intron of the CNBP gene are the direct cause of myotonic dystrophy type 2 (DM2), a multisystem disorder.
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Western Blot
WB result of CNBP Recombinant Rabbit mAb
Primary antibody: CNBP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDa
WB result of CNBP Recombinant Rabbit mAb
Primary antibody: CNBP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDa
WB result of CNBP Recombinant Rabbit mAb
Primary antibody: CNBP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDa
IP
CNBP Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CNBP in 0.4 mg Jurkat whole cell lysate.
Western blot was performed on the immunoprecipitate using CNBP Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP (S0B4085) was used at 1/2000 dilution.
Lane 1: Jurkat whole cell lysate 20 µg (Input)
Lane 2: CNBP Rabbit mAb IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
Predicted MW: 19 kDa
Observed MW: 17 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CNBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-CNBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CNBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CNBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CNBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-CNBP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
