ClpX Recombinant Rabbit mAb (S-4460-33)

Catalog Number: S0B60174 Application: WB, IHC-P Reactivity: Hu, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	ClpX
Synonyms	ATP-dependent clpX-like chaperone, mitochondrial; ATP-dependent Clp protease ATP-binding subunit clpX-like; mitochondrial; Caseinolytic mitochondrial matrix peptidase chaperone subunit XImported; CLPX
Immunogen	Synthetic Peptide
Location	Mitochondrion
Accession	O76031
Clone Number	S-4460-33
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Rt, Mk
Positive Sample	HT-1080, Saos-2, A673, HeLa, rat stomach, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Rt, Mk
IHC-P	1:50	Hu

Background

ClpX is a highly conserved ATP-dependent molecular chaperone belonging to the Clp/Hsp100 protein family, which plays a critical role in protein quality control across various biological systems, including bacteria, mitochondria, and chloroplasts. This protein typically exists as a homohexameric ring structure, with each monomer consisting of approximately 420–450 amino acids, containing an N-terminal zinc-binding domain (ZBD), a central AAA+ ATPase domain, and a C-terminal PDZ domain. The core function of ClpX lies in its ability to specifically recognize aberrant or misfolded proteins carrying SsrA tags, ssrA-like sequences, or specific C-terminal degradation signals, and using the energy generated from ATP hydrolysis, to unfold and translocate these substrate proteins into the proteolytic chamber of its associated ClpP protease for degradation. The ClpXP protease complex formed by ClpX and ClpP is not only responsible for clearing toxic protein aggregates within cells to maintain proteostasis, but also participates in regulating key biological processes such as the cell cycle, gene expression, and stress responses. In medical research, ClpX has been considered a potential target for antimicrobial and anti-tumor agents due to its essentiality in bacterial pathogens (such as Mycobacterium tuberculosis and Staphylococcus aureus) and its homologous function in eukaryotic mitochondria—for example, ADEP-class compounds targeting ClpX have emerged as a research hotspot in the development of novel antibiotics.

Picture

Western Blot

WB result of ClpX Recombinant Rabbit mAb
Primary antibody: ClpX Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-1080 whole cell lysate 20 µg
Lane 2: Saos-2 whole cell lysate 20 µg
Lane 3: A673 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 62 kDa

WB result of ClpX Recombinant Rabbit mAb
Primary antibody: ClpX Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat stomach lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 62 kDa

WB result of ClpX Recombinant Rabbit mAb
Primary antibody: ClpX Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 62 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-ClpX antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-ClpX antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.