WB result of CLPP Recombinant Rabbit mAb
Primary antibody: CLPP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Saos-2 whole cell lysate 20 µg
Lane 2: Hek-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 25 kDa
CLPP Recombinant Rabbit mAb (S-4169-31)
CLPP Recombinant Rabbit mAb (S-4169-31)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CLPP |
| Synonyms | ATP-dependent Clp protease proteolytic subunit, mitochondrial; Caseinolytic mitochondrial matrix peptidase proteolytic subunitImported; Endopeptidase Clp |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q16740 |
| Clone Number | S-4169-31 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Saos-2, HEK-293, mouse liver, mouse brain, mouse liver, mouse stomach, rat liver, rat brain, rat kidney |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu |
Background
CLPP is a highly conserved serine protease located in the mitochondrial matrix that serves as a core component in maintaining mitochondrial protein homeostasis. Its function depends on binding with the ATPase chaperone CLPX to form the CLPXP complex, in which CLPX recognizes and, using ATP hydrolysis energy, unfolds damaged or misfolded protein substrates before translocating them into the proteolytic chamber formed by the double heptameric barrel structure of CLPP, where they are degraded into small peptides—thereby eliminating toxic protein aggregates within mitochondria. This system is broadly conserved across species (for instance, human CLPP consists of 277 amino acids and is localized at chromosomal region 19p13.3). Beyond its physiological functions, aberrations in CLPP are closely linked to various diseases: germline mutations can cause Perrault syndrome type 3, a rare autosomal recessive disorder characterized by sensorineural hearing loss and ovarian insufficiency in females; in cancer biology, CLPP is overexpressed in multiple tumors including multiple myeloma and breast cancer, correlating with poor prognosis, which positions it as a promising anticancer target. Small-molecule agonists (such as ONC201) that induce its hyperactivation can lead to mitochondrial dysfunction and tumor cell apoptosis, while inhibition of its activity can also effectively kill myeloma cells and overcome drug resistance.
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Western Blot
WB result of CLPP Recombinant Rabbit mAb
Primary antibody: CLPP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Lane 4: mouse stomach lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 25 kDa
WB result of CLPP Recombinant Rabbit mAb
Primary antibody: CLPP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 26 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-CLPP antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-CLPP antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CLPP antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CLPP antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CLPP antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
