WB result of Cleaved PARP (Asp214) Recombinant Rabbit mAb
Primary antibody: Cleaved PARP (Asp214) Recombinant Rabbit mAb at 1/1000 dilution overnight at 4°C
Lane 1: THP-1 treated with Cycloheximide (10 μg/mL, 18 hours) whole cell lysate 20 µg
Lane 2: THP-1 treated with Cycloheximide (10 μg/mL, 18 hours), then treated with hTNF-α(20 ng/mL, 4 hours) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 113 kDa
Observed MW: 95 kDa
Cleaved PARP (Asp214) Recombinant Rabbit mAb (S-5860)
Cleaved PARP (Asp214) Recombinant Rabbit mAb (S-5860)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PARP |
| Synonyms | Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1); DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1 (ADPRT 1); Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1; ADPRT; PPOL; PARP1 |
| Location | Cytoplasm, Nucleus |
| Accession | P09874 |
| Clone Number | S-5860 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Mk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Hu, Ms, Mk |
Background
PARP (poly(ADP-ribose) polymerase) is a family of nuclear enzymes that detect and signal DNA strand breaks, catalyzing the transfer of ADP-ribose units from NAD⁺ to target proteins to create poly(ADP-ribose) chains that recruit DNA repair factors, thereby playing a central role in base-excision and single-strand break repair; pharmacological inhibition of PARP1/2 is synthetically lethal in BRCA1/2-mutated tumors because it traps the enzyme on DNA, preventing repair of replication-associated double-strand breaks that cannot be accurately rejoined in homologous-recombination–deficient cells, a strategy that underlies several approved cancer therapies.
Picture
Picture
Western Blot
WB result of Cleaved PARP (Asp214) Recombinant Rabbit mAb
Primary antibody: Cleaved PARP (Asp214) Recombinant Rabbit mAb at 1/1000 dilution overnight at 4°C
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with Etoposide (25uM, 5hours) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 113 kDa
Observed MW: 95 kDa
WB result of Cleaved PARP (Asp214) Recombinant Rabbit mAb
Primary antibody: Cleaved PARP (Asp214) Recombinant Rabbit mAb at 1/1000 dilution overnight at 4°C
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa serum starvd overnight, then treated with Staurosporine (1 μM, 3hours) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 113 kDa
Observed MW: 95 kDa
