WB result of Caspase-9 (Asp330) Recombinant Rabbit mAb
Primary antibody: Caspase-9 (Asp330) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa
Caspase-9 (Asp330) Recombinant Rabbit mAb (S-1531-8)
Caspase-9 (Asp330) Recombinant Rabbit mAb (S-1531-8)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Cleaved Caspase-9 (Asp330) |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P55211 |
| Clone Number | S-1531-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | HeLa |
| Predicted Reactivity | AfGrMk, Gor, Si |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Caspase-9 (Asp330) is the active form of human caspase-9 that has undergone auto-proteolytic cleavage after Asp-330 within the apoptosome, releasing the p35/p12 heterodimer and exposing the catalytic cysteine active site to initiate the intrinsic apoptotic cascade.
Picture
Picture
Western Blot
