WB result of Cleaved Caspase-8 (Asp387) Recombinant Rabbit mAb
Primary antibody: Cleaved Caspase-8 (Asp387) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated CTLL-2 whole cell lysate 20 µg
Lane 2: CTLL-2 treated with 10 μg/ml cycloheximide overnight, then treated with 20 ng/ml mTNF-α for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 18, 43 kDa
Cleaved Caspase-8 (Asp387) Recombinant Rabbit mAb (S-2953)
Cleaved Caspase-8 (Asp387) Recombinant Rabbit mAb (S-2953)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Cleaved Caspase-8 (Asp387) |
| Synonyms | CASP-8; Casp8 |
| Location | Cytoplasm, Nucleus |
| Accession | O89110 |
| Clone Number | S-2953 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Ms |
| ICC | 1:500 | Ms |
Background
Cleaved Caspase-8 (Asp387) is the ~18 kDa C-terminal catalytic fragment generated by autoproteolysis at Asp387 during death-receptor-mediated apoptosis, retaining the active-site cysteine and small subunit that, once released from the DISC and freed from its prodomain, forms a heterotetrameric protease capable of cleaving downstream caspases and substrates such as Bid, thereby amplifying the extrinsic apoptotic signaling cascade.
Picture
Picture
Western Blot
Immunocytochemistry
ICC analysis of RAW264.7 cells treated with Cycloheximide(2.5ug/ml,4h) and mTNF-α (20ng/ml,4h) (top panel) and untreated RAW264.7 cells (below panel). Anti- Cleaved Caspase-8 (Asp387) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
