WB result of Caspase-8 (Asp374) Recombinant Rabbit mAb
Primary antibody: Caspase-8 (Asp374) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 10 μg/ml Cycloheximide for 18 hours, then treated with 20 ng/ml hTNF-α for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 43, 60 kDa
Caspase-8 (Asp374) Recombinant Rabbit mAb (S-1530-30)
Caspase-8 (Asp374) Recombinant Rabbit mAb (S-1530-30)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Cleaved Caspase-8 (Asp374) |
| Synonyms | Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE (FLICE); MCH5; CASP8 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q14790 |
| Clone Number | S-1530-30 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Cleaved Caspase-8 (Asp374) is a key protein involved in the extrinsic apoptosis pathway, which is activated by death receptors such as Fas (CD95) and tumor necrosis factor receptor 1 (TNFR1). When cleaved at Asp374, it generates active fragments, including the p18 subunit, which can further activate downstream effector caspases like caspase-3, -6, and -7, ultimately leading to apoptosis. This protein is also implicated in regulating inflammatory necrotic cell death pathways such as necroptosis and pyroptosis.
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Western Blot
