Claudin18.2 + Claudin18.1 Recombinant Rabbit mAb (SDT-298-29)

Catalog Number: S0B2191 Application: IHC-P,ICC Reactivity: Human Conjugation: Unconjugated Brand: Starter

Price:

$120 USD

Size: 25ul 100μl 500μl

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Product Details

Product Specification

Host	Rabbit
Antigen	CLAUDIN18.2 + CLAUDIN18.1
Synonyms	N/A
Immunogen	Synthetic Peptide
Location	Cytoplasm, Cell membrane
Accession	P56856
Clone Number	SDT-298-29
Antibody Type	Recombinant mAb
Application	IHC-P, ICC
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Tag	N/A
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:1000
ICC	1:500

Background

Claudin 18 is a protein that in humans is encoded by the CLDN18 gene. It belongs to the group of claudins. CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. The claudin-18 gene has two splice variants: claudin 18.1, which is found primarily in the lung, and claudin 18.2, which is expressed exclusively in the stomach.

Picture

Western Blot

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human lung. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol

Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in KATOIII cells. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).