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Claudin 9 Recombinant Rabbit mAb (S-2849-16)

Claudin 9 Recombinant Rabbit mAb (S-2849-16)

Catalog Number: S0B6499 Application: WB, IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen Claudin 9
Synonyms Claudin-9; CLDN9
Immunogen Synthetic Peptide
Location Cell membrane
Accession O95484
Clone Number S-2849-16
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample MCF7
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:250 Hu
IHC-P 1:1000 Hu

Background

Claudin-9 is a 211-amino-acid tetraspan tight-junction protein that seals the paracellular space between epithelial and endothelial cells while selectively controlling ion and solute permeability; it is highly expressed in the inner ear, lung, testis and epidermis, where its absence or mutation causes deafness, skin barrier defects and increased susceptibility to viral entry, and its transcription is driven by grainyhead-like transcription factors that bind a conserved 5′-enhancer, making claudin-9 a critical determinant of tissue-specific barrier integrity, ion homeostasis and innate defense.

Picture

Western Blot

WB result of Claudin 9 Recombinant Rabbit mAb
Primary antibody: Claudin 9 Recombinant Rabbit mAb at 1/250 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 20 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-Claudin 9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pituitary gland. Anti-Claudin 9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human breast. Anti-Claudin 9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Claudin 9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded MCF7 cells. Anti-Claudin 9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded HeLa cells. Anti-Claudin 9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.