WB result of Claudin 1 Recombinant Rabbit mAb
Primary antibody: Claudin 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 18 kDa
Claudin 1 Recombinant Rabbit mAb (SDT-1289-55)
Claudin 1 Recombinant Rabbit mAb (SDT-1289-55)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Claudin 1 |
| Synonyms | Senescence-associated epithelial membrane protein; CLDN1; CLD1, SEMP1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | O95832 |
| Clone Number | SDT-1289-55 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu |
| Positive Sample | MCF7, A431, HepG2, mouse skin, rat kidney |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu |
| IF | 1:200 | Hu |
Background
Claudin 1 is a 23 kDa multi-pass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. It mainly expresses the peripheral neuroma (partial type of negative), derivative skin fibrous sarcoma, fibroma and deity fibroma.
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Western Blot
WB result of Claudin 1 Recombinant Rabbit mAb
Primary antibody: Claudin 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skin lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 18 kDa
WB result of Claudin 1 Recombinant Rabbit mAb
Primary antibody: Claudin 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 18 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Claudin 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Claudin 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Claudin 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human synovialsarcoma. Anti-Claudin 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human dermatofibrosarcoma protuberans. Anti-Claudin 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human colon cancer. Anti-Claudin 1 antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (Alexa Fluor® 647 Conjugate) (S0B4051) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human cervical cancer. Anti-Claudin 1 antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (Alexa Fluor® 647 Conjugate) (S0B4051) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human liver cancer. Anti-Claudin 1 antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) (Alexa Fluor® 647 Conjugate) (S0B4051) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
