WB result of Clathrin heavy chain Recombinant Rabbit mAb
Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 192 kDa
Observed MW: 180 kDa
Clathrin heavy chain Recombinant Rabbit mAb (S-1690-26)
Clathrin heavy chain Recombinant Rabbit mAb (S-1690-26)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Clathrin heavy chain |
| Synonyms | Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17 (CLH-17); CLTC; CLH17; CLTCL2; KIAA0034 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Melanosome |
| Accession | Q00610 |
| Clone Number | S-1690-26 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, SH-SY5Y, HeLa, 293T, Neuro-2a, NIH/3T3, C6, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Clathrin heavy chain (CHC) is a critical component of the clathrin protein complex, which is involved in intracellular trafficking, particularly in clathrin-mediated endocytosis (CME). The major function of neuronal clathrin is to recycle synaptic vesicle proteins following exocytosis. Clathrin heavy chain is probably the limiting component in triskelion biosynthesis, and an increase in the amount of clathrin heavy chain may reflect control of triskelion synthesis. Clathrin-mediated pathways are linked to disease and tissue differentiation. The balance of cargo sequestration into CCVs can affect cell fate, linking clathrin-mediated pathways to disease.
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Picture
Western Blot
WB result of Clathrin heavy chain Recombinant Rabbit mAb
Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 192 kDa
Observed MW: 180 kDa
WB result of Clathrin heavy chain Recombinant Rabbit mAb
Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 192 kDa
Observed MW: 180 kDa
