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CKS1 GST Tag Protein, Human

CKS1 GST Tag Protein, Human

Catalog Number: UA085010 Brand: UA BIOSCIENCE
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$170 USD
$170 USD
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Product Details

Product Specification


Species Human
Synonyms CKS-1
Accession P61024
Amino Acid Sequence

M1-K79 with GST Tag at the N-Terminus
Expression System E.coli
Molecular Weight

30-35kDa (Reducing)
Purity ＞95% by SDS-PAGE & > 90% by HPLC
Conjugation Unconjugated
Tag GST Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Ganoth D, Bornstein G, Ko TK, Larsen B, Tyers M, Pagano M, Hershko A. The cell-cycle regulatory protein Cks1 is required for SCF(Skp2)-mediated ubiquitinylation of p27. Nat Cell Biol. 2001 Mar;3(3):321-4.
2.Sitry D, Seeliger MA, Ko TK, Ganoth D, Breward SE, Itzhaki LS, Pagano M, Hershko A. Three different binding sites of Cks1 are required for p27-ubiquitin ligation. J Biol Chem. 2002 Nov 1;277(44):42233-40.

Background

CKS1 is a 9-kDa accessory protein that binds conserved “CKS” pockets on CDK1 and CDK2. By docking the SCF Skp2 E3 ligase to CDK–cyclin complexes, it licenses ubiquitination of the CDK inhibitor p27Kip1, thereby driving G1→S progression. Elevated CKS1 is frequent in solid tumours and is an independent marker of metastasis and poor prognosis.

Picture

SDS-PAGE

2μg (R: reducing condition, N:non-reducing condition).

SEC-HPLC

The purity of CKS1 GST Tag Protein, Human is more than 90% determined by SEC-HPLC.