WB result of CK1α Recombinant Rabbit mAb
Primary antibody: CK1α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 35 kDa
CK1α Recombinant Rabbit mAb ,PBS Only(SDT-2884-60)
CK1α Recombinant Rabbit mAb ,PBS Only(SDT-2884-60)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CK1α |
| Synonyms | Casein kinase I isoform alpha; CKI-alpha; CK1; CSNK1A1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | P48729 |
| Clone Number | SDT-2884-60 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | PC-3, Caco-2, MCF7, HeLa, NIH/3T3, mouse spleen, rat brain, COS-7 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution,4 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:100 | Hu |
Background
CK1α (casein kinase 1 isoform alpha) is a ubiquitously expressed, constitutively active serine/threonine kinase that docks to a conserved recognition motif (pSer/pThr-X-X-S/T) in hundreds of cytoplasmic, nuclear and membrane substrates, thereby governing Wnt/β-catenin signaling by phosphorylating β-catenin for β-TrCP-mediated degradation, synchronizing circadian rhythms through PER protein turnover, regulating DNA-damage checkpoints by modifying p53 and MDM2, driving hematopoietic stem-cell maintenance via AKT pathway modulation, and serving as an essential, druggable node whose loss-of-function triggers ribosomal stress and selective apoptosis in acute myeloid leukemia cells.
Picture
Picture
Western Blot
WB result of CK1α Recombinant Rabbit mAb
Primary antibody: CK1α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 35 kDa
WB result of CK1α Recombinant Rabbit mAb
Primary antibody: CK1α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 35 kDa
WB result of CK1α Recombinant Rabbit mAb
Primary antibody: CK1α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 35 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-CK1α antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK1α antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CK1α antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CK1α antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
