CHX10 Recombinant Rabbit mAb (S-3940-13)

Catalog Number: S0B6887 Application: IHC-P, IF Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	CHX10
Synonyms	Visual system homeobox 2; Ceh-10 homeodomain-containing homolog; Homeobox protein CHX10; Chx10; Vsx2
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	Q61412
Clone Number	S-3940-13
Antibody Type	Recombinant mAb
Isotype	IgG
Application	IHC-P, IF
Reactivity	Hu, Ms, Rt
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:500	Hu, Ms, Rt
ICC	1:500	Ms, Rt

Background

CHX10, also known as VSX2 (Visual System Homeobox 2), is a paired-like homeodomain transcription factor that serves as a master regulator in vertebrate eye development, playing indispensable roles in the specification, proliferation, and differentiation of retinal progenitor cells into various neuronal lineages, particularly bipolar cells and Müller glia. Expressed primarily in the developing neural retina, CHX10 functions by binding to specific DNA sequences to activate or repress target genes involved in cell cycle control and fate determination, thereby ensuring the proper formation of retinal layers and overall eye morphology. Mutations in the CHX10 gene are associated with severe congenital eye disorders in both humans and animals, such as microphthalmia (abnormally small eyes) and anophthalmia (absence of eyes), highlighting its critical importance in oculogenesis; furthermore, its conserved expression pattern across species underscores its fundamental evolutionary role in establishing the visual system, making it a key marker for studying retinal development and a potential target for regenerative therapies aimed at restoring vision through stem cell-derived retinal tissues.

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Immunohistochemistry

IHC shows positive staining in paraffin-embedded human retina. Anti-CHX10 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse retina. Anti-CHX10 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat retina. Anti-CHX10 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunofluorescence

IF shows positive staining in paraffin-embedded mouse retina. Anti-CHX10 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded rat retina. Anti-CHX10 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.