WB result of CHOP Recombinant Mouse mAb
Primary antibody: CHOP Recombinant Mouse mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 2 µg/ml tunicamycin for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 30 kDa
This blot was developed with high sensitivity substrate
CHOP Recombinant Mouse mAb (S-3924)
CHOP Recombinant Mouse mAb (S-3924)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CHOP |
| Synonyms | DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; C/EBP-homologous protein (CHOP); C/EBP-homologous protein 10 (CHOP-10); CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153; CHOP; CHOP10; GADD153; DDIT3 |
| Location | Cytoplasm, Nucleus |
| Accession | P35638 |
| Clone Number | S-3924 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Tunicamycin treated HeLa |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
CHOP (C/EBP homologous protein) is a 29 kDa, 169-amino-acid bZIP transcription factor whose basic DNA-binding region carries two disruptive proline substitutions that enable it to form non-DNA-binding heterodimers with other C/EBP or ATF4 partners, thereby acting as a dominant-negative repressor of many C/EBP target genes while also partnering with ATF4 to transcriptionally activate pro-apoptotic genes such as GADD34, BIM, DR5 and TRB3, leading—after phosphorylation by p38 MAPK at Ser78/81 and JNK-mediated enhancement—to mitochondrial outer-membrane permeabilization, cytochrome-c release, caspase activation and endoplasmic-reticulum-stress-induced apoptosis.
Picture
Picture
Western Blot
Immunocytochemistry
ICC analysis of HeLa cells treated with tunicamycin (2μg/ml, 8h) (top panel) and untreated HeLa cells (below panel). Anti-CHOP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
