WB result of CHMP6/VPS20 Recombinant Rabbit mAb
Primary antibody: CHMP6/VPS20 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 27 kDa
CHMP6/VPS20 Recombinant Rabbit mAb (S-1702-78)
CHMP6/VPS20 Recombinant Rabbit mAb (S-1702-78)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CHMP6/VPS20 |
| Synonyms | Charged multivesicular body protein 6; Chromatin-modifying protein 6; Vacuolar protein sorting-associated protein 20 (Vps20; hVps20) |
| Immunogen | Synthetic Peptide |
| Location | Endosome |
| Accession | Q96FZ7 |
| Clone Number | S-1702-78 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms, Rt |
| Positive Sample | mouse testis, mouse lung, rat lung |
| Predicted Reactivity | Gor |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:250 | Ms, Rt |
Background
CHMP6, also known as VPS20, is a protein encoded by the human gene CHMP6 and belongs to the charged multivesicular body protein family. It is a core component of the ESCRT-III (endosomal sorting complex required for transport III) complex, which is essential for the formation of multivesicular bodies (MVBs) and the sorting of endosomal cargo proteins into these MVBs. MVBs are involved in the degradation of membrane proteins, such as growth factor receptors, lysosomal enzymes, and lipids, by transporting them to lysosomes. CHMP6 is also known to interact with other components of the ESCRT machinery, such as CHMP4 and members of the ESCRT-II complex, to regulate cargo sorting and membrane remodeling processes. The protein is highly conserved across species and contains specific domains like coiled-coil structures and an N-myristoylation site, which are crucial for its function.
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Western Blot
WB result of CHMP6/VPS20 Recombinant Rabbit mAb
Primary antibody: CHMP6/VPS20 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 25 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse colon. Anti-CHMP6/VPS20 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-CHMP6/VPS20 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-CHMP6/VPS20 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
