WB result of CGRP Recombinant Rabbit mAb
Primary antibody: CGRP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: TT whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 10 kDa
CGRP Recombinant Rabbit mAb (S-2490-74)
CGRP Recombinant Rabbit mAb (S-2490-74)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CGRP |
| Synonyms | Calcitonin gene-related peptide 1; CGRP1; Alpha-type CGRP; Calcitonin gene-related peptide I (CGRP-I); CALC1; CALCA |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P06881 |
| Clone Number | S-2490-74 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | TT |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Hu |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
CGRP (calcitonin gene-related peptide) is a 37-amino-acid neuropeptide produced by tissue-specific splicing of the CALCA gene in primary afferent sensory neurons and central neurons; it signals through a heterodimeric receptor composed of the G-protein-coupled receptor CALCRL and the accessory protein RAMP1, potently dilating cerebral and dermal vasculature, modulating nociceptive transmission, and mediating neuro-inflammatory responses, making it a key target for monoclonal antibodies and small-molecule antagonists in the prevention of migraine and the treatment of cluster headache.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spinal cord. Anti-CGRP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spinal cord (partial view). Anti-CGRP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spinal cord. Anti-CGRP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spinal cord. Anti-CGRP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
