WB result of CEP55 Recombinant Rabbit mAb
Primary antibody: CEP55 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: HT-1080 whole cell lysate 20 µg
Lane 3: SK-MEL-28 whole cell lysate 20 µg
Lane 4: MDA-MB-231 whole cell lysate 20 µg
Lane 5: T-47D whole cell lysate 20 µg
Lane 6: MCF7 whole cell lysate 20 µg
Lane 7: HeLa whole cell lysate 20 µg
Lane 8: Jurkat whole cell lysate 20 µg
Lane 9: HEK-293 whole cell lysate 20 µg
Lane 10: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 55 kDa
CEP55 Recombinant Rabbit mAb (S-3562-33)
CEP55 Recombinant Rabbit mAb (S-3562-33)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CEP55 |
| Synonyms | Centrosomal protein of 55 kDa; C10orf3; URCC6 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | Q53EZ4 |
| Clone Number | S-3562-33 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | LNCaP, HT-1080, SK-MEL-28, MDA-MB-231, T-47D, MCF7, HeLa, Jurkat, HEK-293, HepG2, mouse testis, rat testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
Background
CEP55 (Centrosomal Protein 55), also known as FLJ10540 or C5orf38, is a coiled-coil protein primarily localized to the midbody during the final stages of cell division, where it plays a critical role in cytokinesis and abscission by recruiting essential machinery such as the ESCRT-III complex and ALIX to ensure the proper physical separation of daughter cells. Beyond its canonical function in mitosis, CEP55 is significantly overexpressed in various malignant tumors, including breast, lung, and liver cancers, acting as an oncogene that promotes cell proliferation, migration, and invasion while inhibiting apoptosis, often through interactions with key signaling pathways like PI3K/AKT and MAPK. Due to its restricted expression in normal adult tissues—being largely absent except in testis—and its high prevalence in cancer cells, CEP55 has emerged as a promising tumor-specific antigen for immunotherapy and a potential prognostic biomarker, linking defective cytokinesis directly to genomic instability and tumorigenesis.
Picture
Picture
Western Blot
WB result of CEP55 Recombinant Rabbit mAb
Primary antibody: CEP55 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDa
WB result of CEP55 Recombinant Rabbit mAb
Primary antibody: CEP55 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDa
