WB result of CENPM Recombinant Rabbit mAb
Primary antibody: CENPM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: PANC-1 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 23 kDa
CENPM Recombinant Rabbit mAb (S-1870-36)
CENPM Recombinant Rabbit mAb (S-1870-36)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CENPM |
| Synonyms | Centromere protein M; CENP-M; Interphase centromere complex protein 39; Proliferation-associated nuclear element protein 1; C22orf18; ICEN39; PANE1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q9NSP4 |
| Clone Number | S-1870-36 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Ramos, HeLa, PANC-1, HepG2, RAW264.7, C6 |
| Predicted Reactivity | AfGrMk, Bb, Cz, Gor, Mq, Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
CENPM protein, also known as centromere protein M, is an inner protein of the kinetochore, a multi-protein complex that binds spindle microtubules to regulate chromosome segregation during cell division. It belongs to the constitutive centromere-associated network protein group, whose members interact with outer kinetochore proteins and help maintain centromere identity at each cell division cycle. CENPM is structurally related to GTPases but cannot bind guanosine triphosphate. A point mutation affecting its interaction with CENP-I impairs kinetochore assembly and chromosome alignment, suggesting its requirement for kinetochore formation. CENPM is upregulated in various types of cancer, including glioblastoma multiforme, and is associated with poor prognosis. It plays a role in cell cycle regulation and may contribute to tumor metastasis and recurrence. CENPM is rarely mutated in human cancers, making it a potential prognostic marker and therapeutic target.
Picture
Picture
Western Blot
WB result of CENPM Recombinant Rabbit mAb
Primary antibody: CENPM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 23 kDa
WB result of CENPM Recombinant Rabbit mAb
Primary antibody: CENPM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 23 kDa
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-CENPM antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
