Cell proliferation assay kit (MTS)
Cell proliferation assay kit (MTS)
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Product Specification
| Usage |
Experimental operation: 1. MTS working fluid preparation: Mix MTS Solution and PMS Solution at a ratio of 20: 1 to obtain the MTS working solution. If the prepared MTS working solution needs to be stored, it should be stored at-20 ℃ in the dark from light. At this time, it is recommended to dispense appropriate amounts to avoid repeated freezing and thawing. Note: Both MTS Solution and PMS Solution are easy to decompose when exposed to light, so they need to be protected from light during preparation and use (can be wrapped in tin foil). If you want to store this kit at 4 °C for frequent use, the MTS working solution should be prepared before each test. The prepared unused MTS working solution needs to be stored at-20 °C protected from light. 2. Cell culture: The cells are seeded in a suitable container such as a well plate. For 96-well plates, it is recommended to add 100uL of medium per well, and it is recommended to inoculate 5-100 × 10 per well3Cells. The cells of the experimental group can be treated with the drug of interest for a period of time according to the experimental design, at which time it is necessary to set up a blank group (containing medium only) and a control group (containing medium, cells and drug solvent). Note: The specific number of cells seeded per well can be adjusted according to the specific cell type. 3. MTS incubation: 20 uL of MTS working solution was added to each well and then incubated in a cell incubator for 1-4 h. Note: The MTS incubation time can be adjusted for specific experiments. 4. Detection: The absorbance (A) was measured at 490 nm with a microplate reader. If a 490 nm filter is not available, optionally use a 450-540 nm filter. 5. Data analysis: The cell survival rate is calculated as follows Cell viability (%) = [(AExperimental group-ABlank group)/(AControl group-ABlank group)] ×100 |
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| Description | MTS Cell Proliferation Assay Kit (MTS Cell Proliferation Assay Kit) is a common kit for detecting cell proliferation and toxicity. MTS is a novel tetrazolium salt, which can be reduced by succinate dehydrogenase in mitochondria of living cells to the water-soluble product Formazan. Formazan is specifically absorbed at 490 nm, so cell proliferation can be reflected by measuring the absorbance at 490 nm. The faster the cell proliferation, the higher the absorbance value. Compared with the common MTT kit, this kit does not need to remove the original culture medium, nor does it need to use another solvent to dissolve formazan, which is more convenient to use, and can avoid experimental errors caused by taking away part of formazan when removing the culture medium. This kit is very flexible to use, and incubation can be continued after detection to obtain better color development results. In addition, the kit has the advantages of wide linear range, high sensitivity and safe use. Product Components:
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| PubChem CID | 0 | |||||||||
| General Notes | 1. When the cells are cultured for a long time, the edge wells of the 96-well plate are prone to liquid evaporation. It is recommended to discard the circle around the 96-well plate and add sterile water, culture medium or PBS instead; At the same time, the 96-well plate can be placed in the incubator close to the water source. 2. MTS Solution and PMS Solutin will solidify at low temperatures. Before use, please return to room temperature or 37 °C water bath for about 10 minutes until completely melted before use. 3. MTS Solution and PMS Solution are sensitive to light. If stored at 4 °C, the color of the solution may change slightly. This change may increase the background absorbance value, but it will not affect the final experimental results. 4. If it cannot be detected in time, you can add 25uL of 10% SDS to each well after the incubation in Step 3 to terminate the reaction. The SDS-treated well plate can be placed Store in an incubator protected from light for no more than 18 hours. 5. For your safety and health, please wear a lab coat and disposable gloves. |
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| Storage Temp. | Store the kit at -20°C away from light and moisture, stable for 1 year. For frequent use, the kit can be stored at 4°C away from light and moisture, stable for 6 weeks. |
