WB result of CEACAM6 Rabbit mAb
Primary antibody: CEACAM6 Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: BxPC-3 whole cell lysate 20 µg
Negative control: LNCaP whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 45~120 kDa
CEACAM6 Recombinant Rabbit mAb (S-720-54)
CEACAM6 Recombinant Rabbit mAb (S-720-54)
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$100.00 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CEACAM6 |
| Synonyms | Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CD66c, NCA |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P40199 |
| Clone Number | S-720-54 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC | 1:500 |
Background
Carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen) (CEACAM6) also known as CD66c (Cluster of Differentiation 66c), is a member of the carcinoembryonic antigen (CEA) family. Carcinoembryonic antigen-related cell adhesion molecule (CEACAM) family are transmembrane glycoproteins that belong to the immunoglobulin superfamily. They are involved in several biological processes, such as cell growth, differentiation, cell recognition, immune response, and adhesion [7-10]. Different members of the CEACAM family may have different functions. There are four members of CEACAMs (namely, CEACAM1, CEACAM5, CEACAM6 and CEACAM7) have been found in epithelia. Theses CEACAMs members can mediate several cell signaling pathways and lead to various functions, such as tumor suppression/promotion, angiogenesis, lymphocyte activation, cell cycle and adhesion.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-CEACAM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human stomach. Anti-CEACAM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CEACAM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CEACAM6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
