CEACAM-6/CD66c Fc Chimera Protein, Human
CEACAM-6/CD66c Fc Chimera Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CEACAM-6, CD66c, NCA, CEAL, CEA Cell Adhesion Molecule 6 |
| Accession | P40199-1 |
| Amino Acid Sequence | Lys35-Gly320, with C-terminal Human IgG1 Fc KLTIESTPFNVAEGKEVLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLYGPDVPTISPSKANYRPGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGSYMCQAHNSATGLNRTTVTMITVSGIEGRMDPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| Expression System | HEK293 |
| Molecular Weight | 75-95kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human Fc Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1、Strickland L A. et al. (2009) Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinoma. The Journal of Pathology. 218(3): 380-390. |
Background
CEACAM6 is a glycosylphosphoinosi-tol (GPI)-linked, cell surface protein and a member of the CEA family of proteins. Structurally, this protein has two C2-type Ig domains and a single N-terminal V-type Ig domain and shares close homology with CEACAM1, CEACAM7 and CEACAM8. Function-ally, CEACAM6 has been implicated in cell adhesion, cellular invasiveness, resistance to anoikis and metastatic behaviour of tumour cells, which are properties investigators have previously tried to exploit using various CEACAM6-targeted therapy approaches.
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SDS-PAGE
ELISA
Immobilized Biotinylated CEACAM-8/CD66b His&Avi Tag Protein, Human (Cat. No. UA010698) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind CEACAM-6/CD66c Fc Chimera Protein, Human (Cat. No. UA010274) with EC50 of 0.14-0.22μg/ml.
Immobilized CEACAM-6/CD66c Fc Chimera Protein, Human (Cat. No. UA010274) at 10μg/mL (100μL/well) can bind Human CD66b, His tag (Cat. No. S0A1010) with EC50 of 13.79-17.77ng/mL.
