WB result of CDS2 Recombinant Rabbit mAb
Primary antibody: CDS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 52 kDa
CDS2 Recombinant Rabbit mAb (S-3670-56)
CDS2 Recombinant Rabbit mAb (S-3670-56)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CDS2 |
| Synonyms | Phosphatidate cytidylyltransferase 2; CDP-DAG synthase 2; CDP-DG synthase 2; CDP-diacylglycerol synthase 2 (CDS 2); CDP-diglyceride pyrophosphorylase 2; CDP-diglyceride synthase 2; CTP:phosphatidate cytidylyltransferase 2 |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | O95674 |
| Clone Number | S-3670-56 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HepG2, PC-3, HeLa, NIH/3T3, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:250 | Hu, Ms |
Background
CDP-diacylglycerol synthase 2 (CDS2), also known as CDP-DAG synthase 2, is a critical membrane-bound enzyme located primarily in the endoplasmic reticulum that catalyzes the conversion of phosphatidic acid and cytidine triphosphate (CTP) into cytidine diphosphate-diacylglycerol (CDP-DAG), serving as the committed and rate-limiting step in the de novo biosynthesis of major phospholipids such as phosphatidylinositol, phosphatidylglycerol, and cardiolipin. As one of two isoforms in mammals (the other being CDS1), CDS2 plays an indispensable role in maintaining cellular membrane integrity, facilitating intracellular signaling pathways dependent on phosphoinositides, and regulating lipid homeostasis, with its expression and activity being tightly linked to metabolic processes where dysregulation has been associated with various pathologies including lipodystrophy, insulin resistance, and altered susceptibility to viral infections due to its involvement in modifying host membrane composition required for viral replication.
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Picture
Western Blot
WB result of CDS2 Recombinant Rabbit mAb
Primary antibody: CDS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 52 kDa
WB result of CDS2 Recombinant Rabbit mAb
Primary antibody: CDS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 52 kDa
WB result of CDS2 Recombinant Rabbit mAb
Primary antibody: CDS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 52 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-CDS2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDS2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CDS2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
