WB result of CDS1 Recombinant Rabbit mAb
Primary antibody: CDS1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A549 whole cell lysate 20 µg
Lane 2: unboiled SK-BR-3 whole cell lysate 20 µg
Low expression control: unboiled A549 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
CDS1 Recombinant Rabbit mAb (S-3671-30)
CDS1 Recombinant Rabbit mAb (S-3671-30)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CDS1 |
| Synonyms | Phosphatidate cytidylyltransferase 1; CDP-DAG synthase 1; CDP-DG synthase 1; CDP-diacylglycerol synthase 1 (CDS 1); CDP-diglyceride pyrophosphorylase 1; CDP-diglyceride synthase 1; CTP:phosphatidate cytidylyltransferase 1; CDS |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | Q92903 |
| Clone Number | S-3671-30 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SK-BR-3, A431, PC-3, RAW264.7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
CDP-diacylglycerol synthase 1 (CDS1), also known as CDP-DAG synthase 1, is a critical integral membrane enzyme primarily located in the endoplasmic reticulum that catalyzes the conversion of phosphatidic acid (PA) and cytidine triphosphate (CTP) into cytidine diphosphate-diacylglycerol (CDP-DAG), serving as the committed and rate-limiting step in the de novo biosynthesis of major phospholipids such as phosphatidylinositol (PI), phosphatidylglycerol (PG), and cardiolipin. As a central hub in lipid metabolism, CDS1 regulates the flux of PA toward phospholipid production rather than triglyceride synthesis or signaling pathways, thereby maintaining cellular membrane integrity, facilitating signal transduction cascades like the PI3K/Akt pathway, and supporting mitochondrial function through cardiolipin generation; its activity is tightly regulated by feedback inhibition from downstream products and transcriptional mechanisms, and dysregulation or mutations in the CDS1 gene have been implicated in severe metabolic disorders, including congenital lipodystrophy, insulin resistance, and altered susceptibility to viral infections due to compromised membrane architecture.
Picture
Picture
Western Blot
WB result of CDS1 Recombinant Rabbit mAb
Primary antibody: CDS1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A431 whole cell lysate 20 µg
Lane 2: unboiled PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
WB result of CDS1 Recombinant Rabbit mAb
Primary antibody: CDS1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
