WB result of CDKN2A/p16INK4a Recombinant Rabbit mAb
Primary antibody: CDKN2A/p16INK4a Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 50 µg
Lane 2: A20 whole cell lysate 50 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 17-22 kDa
CDKN2A/p16INK4a Recombinant Rabbit mAb (S-2536-38)
CDKN2A/p16INK4a Recombinant Rabbit mAb (S-2536-38)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | p16INK4a |
| Synonyms | Cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A (CDK4I); p16-INK4a (p16-INK4); P16ink4a; Cdkn2a |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | P51480 |
| Clone Number | S-2536-38 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Ms |
| Positive Sample | A20 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Ms |
| ICC | 1:100 | Ms |
Background
p16INK4a is a 16 kDa cyclin-dependent kinase inhibitor encoded by the CDKN2A locus on chromosome 9p21 that specifically binds to and inactivates CDK4/6 complexes, thereby preventing phosphorylation of the retinoblastoma protein (pRb) and enforcing a G1 cell-cycle arrest that functions as a critical tumor-suppressive barrier against oncogenic stress; its expression rises sharply with aging and acts as a robust biomarker of cellular senescence across diverse tissues, while its frequent inactivation through deletion, mutation, or promoter hypermethylation is observed in numerous human cancers, underscoring its pivotal role in both aging-related pathophysiology and oncogenesis.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in A20 cells (top panel) and negative staining in NIH/3T3 cells (below panel). Anti- CDKN2A/p16INK4a antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibod at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
