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CDKN2A/p16 INK4A Recombinant Rabbit mAb (S-4676)

CDKN2A/p16 INK4A Recombinant Rabbit mAb (S-4676)

Catalog Number: S0B6772 Application: WB, IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen CDKN2A/p16 INK4A
Synonyms Tumor suppressor ARF; Alternative reading frame (ARF); Cyclin-dependent kinase inhibitor 2AImported; p14ARF; CDKN2; MLM; CDKN2A
Location Nucleus, Mitochondrion
Accession Q8N726
Clone Number S-4676
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample HeLa, HEK-293, Saos-2
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu
IHC-P 1:1000 Hu

Background

CDKN2A/p16INK4A is a critical tumor suppressor protein encoded by the CDKN2A gene that functions as a key negative regulator of the cell cycle by specifically inhibiting cyclin-dependent kinases 4 and 6 (CDK4/6), thereby preventing the phosphorylation of the retinoblastoma protein (Rb) and arresting cell progression from the G1 to the S phase to curb uncontrolled proliferation. Often referred to as p16, this protein acts as a vital checkpoint against oncogenic transformation, and its loss of function through genetic deletion, mutation, or epigenetic silencing is a hallmark event in numerous human cancers, including melanoma, pancreatic ductal adenocarcinoma, and glioblastoma; conversely, its overexpression is frequently observed in cells undergoing senescence or in HPV-associated malignancies where it serves as a reliable diagnostic biomarker due to the disruption of the Rb pathway by viral oncoproteins, making p16INK4A not only a fundamental component of cellular defense mechanisms but also a significant target for therapeutic strategies and prognostic assessments in oncology.

Picture

Western Blot

WB result of CDKN2A/p16 INK4A Recombinant Rabbit mAb
Primary antibody: CDKN2A/p16 INK4A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg 
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: Saos-2 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows weak staining in paraffin-embedded human breast. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cervix. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti- CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows weak staining in paraffin-embedded human colon. Anti- CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows weak staining in paraffin-embedded human bladder cancer. Anti- CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.