The CDK9/CyclinT1 protein was incubated with ATP and substrate at 25 °C for 60 minutes. Following the incubation, ADP-Glo reagent was added to the reaction mixture. The luminescent signal (RLU) was finally measured using a BMG microplate reader.
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Cyclin-dependent kinase 9/CyclinT1 |
| Accession | CDK9: P50750、CyclinT1: O60563-1 |
| Amino Acid Sequence | CDK9: M1-F372(end), N-terminal GST tag; |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | CDK9: 68.6 kDa; Cyclin T1: 107.8 kDa |
| Purity | > 75% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag, GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris, 300 mM NaCl, 20 mM GSH, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
