WB result of CDK9 Recombinant Rabbit mAb
Primary antibody: CDK9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HL-60 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37, 50 kDa
CDK9 Recombinant Rabbit mAb (S-2661-10)
CDK9 Recombinant Rabbit mAb (S-2661-10)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CDK9 |
| Synonyms | Cyclin-dependent kinase 9; Cell division protein kinase 9; Cdk9 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | Q99J95 |
| Clone Number | S-2661-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HT-29, HeLa, HL-60, HEK-293, NIH/3T3, RAW264.7, mouse testis, mouse liver, PC-12, C6, rat testis, rat liver |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
CDK9 (cyclin-dependent kinase 9) is the catalytic subunit of the positive transcription elongation factor b (P-TEFb), which phosphorylates the RNA polymerase II C-terminal domain and negative elongation factors to promote productive elongation of RNA synthesis; in its activated form CDK9 is complexed with cyclin T or cyclin K and is essential for expression of most RNA polymerase II-dependent genes, and because it controls transcriptional elongation of key regulators of cell proliferation and survival—especially MCL-1, MYC and cyclin D1—CDK9 is an attractive therapeutic target in cancer, inflammation and viral infections, with several ATP-competitive inhibitors such as flavopiridol, dinaciclib and alvocidib already in clinical trials.
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Western Blot
WB result of CDK9 Recombinant Rabbit mAb
Primary antibody: CDK9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Lane 4: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37, 50 kDa
WB result of CDK9 Recombinant Rabbit mAb
Primary antibody: CDK9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat testis lysate 20 µg
Lane 4: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37, 50 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
