CDK6 Recombinant Rabbit mAb (S-4090-71)

Catalog Number: S0B6969 Application: WB, IHC-P Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	CDK6
Synonyms	Cyclin-dependent kinase 6; CR2 protein kinase (CRK2); Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE; Cdkn6; Crk2; Cdk6
Immunogen	Synthetic Peptide
Location	Cytoplasm, Cytoskeleton, Nucleus
Accession	Q64261
Clone Number	S-4090-71
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Ms, Rt
Positive Sample	RAW264.7, mouse testis, mouse spleen, mouse thymus, rat testis, rat spleen, rat thymus
Predicted Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Ms, Rt
IHC-P	1:500	Ms, Rt

Background

Cyclin-dependent kinase 6 (CDK6) is a serine/threonine protein kinase that plays a pivotal role in regulating the cell cycle, specifically during the G1 to S phase transition, by forming active heterodimeric complexes with D-type cyclins (Cyclin D1, D2, or D3). Upon binding to these cyclins, the CDK6 complex phosphorylates the retinoblastoma protein (Rb), which leads to the release of E2F transcription factors and subsequently drives the expression of genes required for DNA synthesis and cell proliferation. Beyond its canonical function in cell cycle control, CDK6 is implicated in various physiological processes including hematopoiesis, differentiation, and metabolism, and its dysregulation—often through gene amplification or overexpression—is frequently observed in numerous cancers, such as breast cancer, lymphoma, and glioblastoma, making it a significant therapeutic target for selective CDK4/6 inhibitors like palbociclib, ribociclib, and abemaciclib, which are widely used in clinical settings to halt tumor growth by inducing G1 cell cycle arrest.

Picture

Western Blot

WB result of CDK6 Recombinant Rabbit mAb
Primary antibody: CDK6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Lane 4: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa

WB result of CDK6 Recombinant Rabbit mAb
Primary antibody: CDK6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Lane 3: rat thymus lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse colon. Anti-CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat colon. Anti-CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.