CDK6-Knockdown HeLa Cell Lysate
CDK6-Knockdown HeLa Cell Lysate
Product Details
Product Details
Product Specification
| Host | Human |
| Mutations | Mutation in exon1 |
| Application | WB |
| Concentration | 2 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | Supplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 1% v/v β-Mercaptoethanol, 0.01% w/v bromophenol blue or phenol red. |
| Stability & Storage | -20 °C for 1 months under sterile conditions; |
Background
CDK6 (cyclin-dependent kinase 6) is a protein kinase involved in cell cycle regulation, with a molecular weight of approximately 40 kDa. CDK6 is expressed in various tissues including lymphoid tissues and the brain. It is activated by binding to D-type cyclins, and its core function is to phosphorylate the retinoblastoma protein, thereby relieving its block on the cell cycle and driving the transition from the G1 phase to the S phase. In addition to this classic role in promoting cell cycle progression, CDK6 also regulates processes such as cell differentiation and apoptosis by phosphorylating other substrates. Due to its dysregulated activity being closely associated with various cancers, CDK6 has become an important anticancer target. CDK4/6 inhibitors targeting it have been successfully applied in clinical practice, but resistance mechanisms such as CDK6 protein overexpression remain a major challenge in current treatment.
Components
