CDK4 Rabbit Polyclonal Antibody

Catalog Number: S0B6829 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

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$70 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	CDK4
Synonyms	Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	P11802
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, MCF7, Jurkat, Neuro-2a, C2C12, CTLL-2, NIH/3T3, MIN6, C6, COS-7
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt, Mk
IHC-P	1:250	Hu, Ms

Background

Cyclin-dependent kinase 4 (CDK4) is a serine/threonine protein kinase that serves as a critical regulator of the G1-to-S phase transition in the eukaryotic cell cycle by forming an active complex with D-type cyclins (D1, D2, or D3). Upon activation, the CDK4-cyclin D complex phosphorylates the retinoblastoma protein (pRb), causing it to release the E2F family of transcription factors, which subsequently drive the expression of genes essential for DNA replication and cell division. The activity of CDK4 is tightly controlled by various mechanisms, including binding to inhibitors like p16INK4a and post-translational modifications, ensuring that cell proliferation occurs only under appropriate physiological conditions. Due to its pivotal role in promoting uncontrolled cell growth when dysregulated, CDK4 is frequently overexpressed or amplified in numerous cancers, making it a significant therapeutic target for selective small-molecule inhibitors used in treating malignancies such as hormone receptor-positive breast cancer.

Picture

Western Blot

WB result of CDK4 Rabbit pAb
Primary antibody: CDK4 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa

WB result of CDK4 Rabbit pAb
Primary antibody: CDK4 Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: CTLL-2 whole cell lysate 20 µg
Lane 4: NIH/3T3 whole cell lysate 20 µg
Lane 5: MIN6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa

WB result of CDK4 Rabbit pAb
Primary antibody: CDK4 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa

WB result of CDK4 Rabbit pAb
Primary antibody: CDK4 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-CDK4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CDK4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse stomach. Anti-CDK4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.