CDK3/CycE1 Protein

Catalog Number: UA080057 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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$1,001.00 SGD

Size: 10μg 100μg 1mg

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Product Details

Product Specification

Species	Human
Accession	Q00526、P24864-1
Amino Acid Sequence	CDK3: Q00526, M1-H305 MDMFQKVEKIGEGTYGVVYKAKNRETGQLVALKKIRLDLEMEGVPSTAIREISLLKELKHPNIVRLLDVVHNERKLYLVFEFLSQDLKKYMDSTPGSELPLHLIKSYLFQLLQGVSFCHSHRVIHRDLKPQNLLINELGAIKLADFGLARAFGVPLRTYTHEVVTLWYRAPEILLGSKFYTTAVDIWSIGCIFAEMVTRKALFPGDSEIDQLFRIFRMLGTPSEDTWPGVTQLPDYKGSFPKWTRKGLEEIVPNLEPEGRDLLMQLLQYDPSQRITAKTALAHPYFSSPEPSPAARQYVLQRFRH CycE1:P24864,M1-A410 MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA
Expression System	Baculovirus-InsectCells
Molecular Weight	CDK3: 61.7 kDa; CycE1: 48.1 kDa
Purity	>90% by SDS-PAGE
Endotoxin	<1EU/μg
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	50mM Tris, 150mM NaCl, 1mM DTT, 20 mM reduced glutathione, 10% glycerol, 0.05% Brij35, pH7.5
Stability & Storage	-80℃

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Bioactivity

CDK3/CycE1 titration in ADP-Glo assay

The CDK3-CycE1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK3-CycE1 protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.