The CDK2/CyclinE1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK2/CyclinE1 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
CDK2/CyclinE1 Protein,Human
CDK2/CyclinE1 Protein,Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P24941-1、P24864-1 |
| Amino Acid Sequence | CDK2: M1-L298(end); |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | CDK2: 34.1 kDa; CyclinE1: 47.2 kDa |
| Purity | > 95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
SEC-HPLC
