CDK2/CycE1 Protein

Catalog Number: UA080055 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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$767.00 USD

Size: 10μg 100μg 1mg

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Product Details

Product Specification

Species	Human
Accession	P24941-1、P24864-1
Amino Acid Sequence	CDK2:M1-L298(end) MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL CycE1:M1-A410(end) MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA
Expression System	Baculovirus-InsectCells
Molecular Weight	CDK2: 60.5 kDa; CycE1: 73.7 kDa
Purity	>95% by SDS-PAGE
Endotoxin	<1EU/μg
Conjugation	Unconjugated
Tag	GST Tag
Physical Appearance	Liquid
Storage Buffer	20mM Tris, 150mM Nacl,1mM DTT, 10% glycerol, pH7.4
Stability & Storage	-80℃

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Bioactivity

CDK2/CycE1 titration in ADP-Glo assay

The CDK2-CycE1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK2-CycE1 protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.