CDK1/CycE1 Protein

Catalog Number: UA080051 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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$1,001.00 SGD

Size: 10μg 100μg 1mg

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Product Details

Product Specification

Species	Human
Accession	P06493-1、P24864-1
Amino Acid Sequence	CDK1 ：M1-M297(end)MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM CycE1： M1-A410(end)MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA
Expression System	Baculovirus-InsectCells
Molecular Weight	CDK1: 60.7 kDa; CycE1: 73.7 kDa
Purity	>90% by SDS-PAGE
Endotoxin	<1EU/μg
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	50mM Hepes, 150mM Nacl, 20mM Reduced Glu, 1mM DTT, 10% glycerol, pH7.5
Stability & Storage	-80℃

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Bioactivity

CDK1/CycE1 titration in ADP-Glo assay

The CDK1-CycE1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK1-CycE1 protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.