CDK13/Cyclin K titration in ADP-Glo assay
CDK13/Cyclin K Protein
CDK13/Cyclin K Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | Q14004-1、O75909-3 |
| Amino Acid Sequence | Q673-S1054/M1-S300 |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | CDK13: 70.3 kDa; Cyclin K: 37.2 kDa |
| Purity | >85% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH 7.5 |
| Stability & Storage | -80℃ |
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Bioactivity
The CDK13/Cyclin K activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK13/Cyclin K protein , ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
SDS-PAGE
SEC-HPLC
