WB result of CDH17 Recombinant Rabbit mAb
Primary antibody: CDH17 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: LoVo whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Negative control: PANC-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 120 kDa
CDH17 Recombinant Rabbit mAb (SDT-3276-46)
CDH17 Recombinant Rabbit mAb (SDT-3276-46)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CDH17 |
| Synonyms | Cadherin-17; Intestinal peptide-associated transporter HPT-1; Liver-intestine cadherin (LI-cadherin) |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q12864 |
| Clone Number | SDT-3276-46 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | LoVo, Caco-2 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
CDH17, also known as liver-intestine cadherin (LI-cadherin), is a unique calcium-dependent transmembrane glycoprotein primarily and specifically expressed in the epithelial cells of the gastrointestinal tract and pancreatic ducts. Structurally, its extracellular region contains seven cadherin repeat domains (EC1-EC7), but its cytoplasmic tail is extremely short, consisting of only about 20 amino acids, preventing it from binding to α/β-catenin like classical cadherins. Functionally, CDH17 plays a dual role: it acts as a homophilic cell adhesion molecule maintaining the structural integrity of the intestinal epithelium, and also functions as a proton-dependent peptide transporter involved in the absorption of oral peptide drugs. Furthermore, in various gastrointestinal cancers (such as colorectal cancer, gastric cancer, and hepatocellular carcinoma), CDH17 is frequently overexpressed and promotes tumor cell proliferation, invasion, and metastasis by activating signaling pathways such as Wnt/β-catenin and MAPK. Consequently, it is regarded as an important cancer diagnostic biomarker and a highly promising therapeutic target. Currently, bispecific antibodies and CAR-T cell therapies targeting CDH17 have demonstrated significant anti-tumor effects in preclinical studies.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Caco-2 cells (top panel) and negative staining in PANC-1 cells (below panel). Anti- CDH17 antibody was used at 1/××× dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
