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CDCP1 Recombinant Rabbit mAb (S-3437-221)

CDCP1 Recombinant Rabbit mAb (S-3437-221)

Catalog Number: S0B60289 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen CDCP1
Synonyms CUB domain-containing protein 1; Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135 kDa protein (SIMA135); Transmembrane and associated with src kinases; CD318; TRASK
Immunogen Recombinant Protein
Location Secreted, Cell membrane
Accession Q9H5V8
Clone Number S-3437-221
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample PC-3, HCT 116, PANC-1, A549
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:500-1:1000 Hu
IHC-P 1:2000 Hu

Background

CDCP1 (CUB domain-containing protein 1), also known as CD318 or SIMA135, is a type I single-pass transmembrane glycoprotein characterized by three CUB domains in its extracellular region and tyrosine phosphorylation sites in its intracellular domain that interact with Src family kinases. In normal tissues, it is primarily expressed in skeletal muscle, colon, and certain stem or progenitor cell populations, but its most prominent function has been observed in multiple human cancers. As a substrate for signaling molecules such as Src kinases, CDCP1, upon tyrosine phosphorylation, initiates downstream signaling pathways that promote tumor cell migration, invasion, anoikis resistance, and metastasis. Recent studies have revealed more complex mechanisms, such as its role as a novel co-receptor for TGF-β1, enhancing the pro-tumorigenic TGF-β/Smad signaling pathway; it has also been found to drive malignant tumor progression through activation of signaling axes such as Wnt/β-catenin. Its high expression in various solid tumors, including lung, colorectal, and breast cancers, is closely associated with poor patient prognosis, making it a highly promising anti-tumor therapeutic target and cancer diagnostic biomarker.

Picture

Western Blot


WB result of CDCP1 Recombinant Rabbit mAb
Primary antibody: CDCP1 Recombinant Rabbit mAb Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: PANC-1 whole cell lysate 20 µg
Lane 5: A431 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 93 kDa
Observed MW: 70, 140 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDCP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-CDCP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human brain. Anti-CDCP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-CDCP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.