IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD98/SLC3A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
CD98/SLC3A2 Rabbit Polyclonal Antibody
CD98/SLC3A2 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD98/SLC3A2 |
| Synonyms | Amino acid transporter heavy chain SLC3A2; 4F2 cell-surface antigen heavy chain (4F2hc); 4F2 heavy chain antigen; Lymphocyte activation antigen 4F2 large subunit; Solute carrier family 3 member 2; MDU1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane, Melanosome |
| Accession | P08195 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | IHC-P, ICC |
| Reactivity | Hu |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
CD98 (Cluster of Differentiation 98) is a heterodimeric transmembrane glycoprotein composed of a heavy chain (CD98hc, encoded by SLC3A2) and a light chain (e.g., LAT1, encoded by SLC7A5), connected by disulfide bonds. It is widely expressed in various cell types, including immune cells, epithelial cells, and tumor cells. Functionally, CD98 is primarily involved in amino acid transport, particularly the transmembrane movement of neutral amino acids, which are essential for cell proliferation and metabolism. Additionally, CD98 interacts with integrin β1 to regulate cell adhesion, migration, and signaling. In the immune system, it plays a role in lymphocyte proliferation and activation, as well as in inflammatory responses. In pathological contexts, CD98 is highly expressed in various cancers and is associated with tumor invasion, metastasis, and drug resistance. For example, in extranodal natural killer/T-cell lymphoma (ENKTL), CD98 enhances chemoresistance via exosome-mediated mechanisms. High expression of CD98 in solid tumors is also linked to poor prognosis and may serve as a biomarker for tumor progression and treatment response. Given its critical role in tumor metabolism and signaling, CD98 has emerged as a potential therapeutic target for cancer.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-CD98/SLC3A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CD98/SLC3A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-CD98/SLC3A2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Molt-4 cells. Anti- CD98/SLC3A2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
