Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cells) labelling CD94 antibody at 1/50 dilution (1 μg) / (Right panel) compared with a Rabbit IgG Isotype Control / (Left panel). Goat Anti-Rabbit IgG DyLight 488 was used as the secondary antibody. Then cells were stained with CD56 - Allophycocyanin Antibody separately.
CD94 Recombinant Rabbit mAb (S-2616-17)
CD94 Recombinant Rabbit mAb (S-2616-17)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD94 |
| Synonyms | Natural killer cells antigen CD94; KP43; Killer cell lectin-like receptor subfamily D member 1; NK cell receptor; KLRD1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q13241 |
| Clone Number | S-2616-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, FCM |
| Reactivity | Hu |
| Positive Sample | NK-92, Human PBMC |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:100 | Hu |
| FCM | 1:50 | Hu |
Background
CD94, encoded by KLRD1, is a type II transmembrane C-type lectin glycoprotein expressed predominantly on natural killer (NK) cells and subsets of CD8⁺ T cells, where it covalently assembles with NKG2A, C, E or F subunits to form disulfide-linked heterodimers that recognize the non-classical MHC-I molecule HLA-E (or Qa-1 in mice) and deliver either inhibitory or activating signals that finely tune cytotoxicity, cytokine secretion and survival of effector lymphocytes, thereby playing a central role in innate and adaptive immunity, tumor immune evasion, viral defense and pregnancy tolerance.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in NK-92 cells (top panel) and negative staining in Jurkat cells (below panel). Anti-CD94 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
