CD86 Recombinant Rabbit mAb (S-6175)

Catalog Number: S0B60528 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	CD86
Location	Cell membrane
Accession	P42082
Clone Number	S-6175
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	Daudi, Raji
Predicted Reactivity	Mk
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:250	Hu

Background

CD86, also historically referred to as B7-2, is a type I transmembrane protein belonging to the immunoglobulin superfamily that serves as a critical costimulatory molecule primarily expressed on the surface of professional antigen-presenting cells (APCs) such as dendritic cells, macrophages, and B cells. Functioning as a ligand for the CD28 and CTLA-4 (CD152) receptors on T cells, CD86 plays a pivotal role in regulating the adaptive immune response by providing the necessary "second signal" required for naive T cell activation, proliferation, and differentiation upon engagement with CD28, while its interaction with CTLA-4 delivers an inhibitory signal that helps maintain immune tolerance and prevent autoimmunity. Although it shares functional redundancy with its counterpart CD80 (B7-1), CD86 is typically constitutively expressed at low levels and is upregulated more rapidly than CD80 following APC activation, making it the dominant costimulatory molecule during the early phases of immune responses, which underscores its significance as a therapeutic target in various clinical contexts, including cancer immunotherapy, autoimmune diseases, and transplant rejection management.

Picture

Western Blot

WB result of CD86 Recombinant Rabbit mAb
Primary antibody: CD86 Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Lane 4: Raji whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate, HT-29 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 55-85 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD86 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-CD86 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-CD86 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.