WB result of CD86 Recombinant Rabbit mAb
Primary antibody: CD86 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: J774A.1 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Negative control: Neuro-2a whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 60-90 kDa
CD86 Recombinant Rabbit mAb (S-4222)
CD86 Recombinant Rabbit mAb (S-4222)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD86 |
| Synonyms | T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD28LG2 |
| Location | Cell membrane |
| Accession | P42081 |
| Clone Number | S-4222 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms |
| Positive Sample | J774A.1, mouse spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:100 | Ms |
Background
CD86, also known as B7-2, is a co-stimulatory molecule expressed on the surface of antigen-presenting cells (APCs) such as dendritic cells, macrophages, and B cells. It plays a crucial role in the immune response by interacting with CD28 on T cells to provide a necessary co-stimulatory signal for T cell activation, proliferation, and differentiation. Additionally, CD86 can also bind to CTLA-4, an inhibitory receptor on T cells, thereby regulating immune responses and maintaining immune homeostasis. This dual functionality makes CD86 a key target in immunotherapy research, particularly in the development of cancer treatments and autoimmune disease management strategies.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD86 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-CD86 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse kidney. Anti-CD86 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
