CD8α Recombinant Rabbit mAb (S-6201)

Catalog Number: S0B60527 Application: WB, IHC-P, IF Reactivity: Ms Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	CD8α
Location	Cell membrane
Accession	P01731
Clone Number	S-6201
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, IF
Reactivity	Ms
Positive Sample	mouse thymus, mouse spleen
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Ms
IHC-P	1:500	Ms
IF	1:500	Ms

Background

CD8α is a crucial immune glycoprotein that serves as a co-receptor for the MHC I-peptide complex, facilitating the recognition of antigens presented by APCs (antigen-presenting cells) to T cells. It is a key component of the CD8 receptor complex, which is predominantly found on cytotoxic T lymphocytes (CTLs). CD8α can form either an α/β heterodimer with CD8β or an α/α homodimer, both of which play essential roles in CTL activation and function. By interacting with the TCR (T cell receptor) and MHC I molecules, CD8α helps recruit the LCK kinase, initiating multiple signaling pathways that drive the CTL-mediated immune response.

Picture

Western Blot

WB result of CD8α Recombinant Rabbit mAb
Primary antibody: CD8α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse thymus lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 33-38 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse spleen. Anti- CD8α antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse testis. Anti- CD8αantibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunofluorescence

IF shows positive staining in paraffin-embedded mouse spleen. Anti-CD8α antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded mouse thymus. Anti-CD8α antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.