CD8+ T cells were enriched from or depleted of splenocytes isolated from C57BL/6 mice. Subsequently, fluorescent staining was performed using anti-CD8 (clone: 53-6.7) Alexa Fluor® 647, and the live cell population was gated for analysis.
CD8 Nanobeads, mouse（RUO）
CD8 Nanobeads, mouse（RUO）
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Product Details
Product Details
Product Specification
| Format | Dextran-coated iron tetroxide magnetic nanoparticles conjugated with anti-mouse CD8 monoclonal antibody |
| Capacity |
2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, and can perform up to 200 sorting runs 1 mL magnetic beads: suitable for a total cell count of 1 × 10⁹, and can perform up to 100 sorting runs |
| Principle of Separation | CD8⁺ cells are magnetically labeled using CD8 Nanobeads, mouse. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD8⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD8⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD8⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive fraction containing CD8⁺ cells must be further separated through a second separation column. |
| Reactivity | Mouse |
| Magnetic Bead Size | 20 nm |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
CD8 NanobeadsThese beads are designed for positive selection or depletion of murine cytotoxic T cells from lymphoid organs, blood, or tissues. The magnetic beads react with the immunoglobulin-like domain of the CD8α chain. The CD8α chain is expressed as a heterodimer with CD8β on cytotoxic T cells that express either the T cell receptor (TCR) α/β or TCR γ/δ, as well as on most thymocytes. As a homodimer composed of two CD8α chains, the CD8 antigen is present on most NK cells, the majority of intraepithelial lymphocytes in the intestine, some activated CD4+CD8+ T cells, and on CD8+ T cells in thymus-deficient mice.
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Validation Data
