CD8+ T cells are enriched or depleted from human PBMCs using CD8 Nanobeads, S or L Separation Columns, and a magnetic separator. Subsequently, fluorescent staining is performed with anti-CD8 (clone: OKT8) Alexa Fluor® 488, and the live cell population is gated for analysis.
CD8 Nanobeads, human（RUO）
CD8 Nanobeads, human（RUO）
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$300 USD
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Product Details
Product Details
Product Specification
| Format | Dextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD8 monoclonal antibody |
| Capacity |
2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations 1 mL magnetic beads: suitable for 5×108total cells, and can perform up to 50 separations |
| Principle of Separation | CD8⁺ cells are magnetically labeled using CD8 Nanobeads for humans. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD8⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD8⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD8⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD8⁺ cells must be further separated using a second column. |
| Reactivity | Human |
| Magnetic Bead Size | 20 nm |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
CD8 antigen forms a complex with the T-cell receptor and serves as a co-molecule on CD8+ cytotoxic T cells, assisting the TCR heterodimer in recognizing MHC class I–peptide complexes. The CD8 molecule consists of an α/β heterodimer or an α/α homodimer. It is highly expressed on cytotoxic T cells and weakly expressed on some NK cells. CD8 is present on most thymocytes and approximately one-third of peripheral blood T cells. CD8+ cytotoxic T cells play a crucial role in eliminating virus-infected cells and tumor cells.
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Validation Data
