IF shows positive staining in paraffin-embedded human tonsil. Anti-CD8α (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
CD8α Mouse mAb (Alexa Fluor® 488 Conjugate) (SDT-1036-34)
CD8α Mouse mAb (Alexa Fluor® 488 Conjugate) (SDT-1036-34)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD8α |
| Synonyms | T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a; MAL |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P01732 |
| Clone Number | SDT-1036-34 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:500 | Hu |
Background
CD8α is a crucial subunit of the CD8 co-receptor complex found on the surface of cytotoxic T lymphocytes (CTLs) and some natural killer (NK) cells. It is a 32 kDa glycoprotein that can form either an α/β heterodimer or an α/α homodimer with CD8β. CD8α plays a vital role in immune responses by binding to MHC class I molecules on antigen-presenting cells (APCs), facilitating the recognition and destruction of infected or abnormal cells. This interaction helps activate the T cell receptor (TCR) complex, recruiting the LCK kinase and initiating multiple signaling pathways. Additionally, CD8αα homodimers are found on intraepithelial lymphocytes (IELs), which protect mucosal surfaces like the intestinal barrier.
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Immunofluorescence
IF shows positive staining in paraffin-embedded Hodgkin’s lymphoma. Anti-CD8α (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
