IF shows positive staining in paraffin-embedded human tonsil. Anti-CD79a/MB-1 (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
CD79a/MB-1 Recombinant Rabbit mAb (Alexa Fluor® 594 Conjugate) (S-030-18)
CD79a/MB-1 Recombinant Rabbit mAb (Alexa Fluor® 594 Conjugate) (S-030-18)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD79a/MB-1 |
| Synonyms | Ig-alpha, Surface IgM-associated protein, IGA |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P11912 |
| Clone Number | S-030-18 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 594 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:500 | null |
Background
The CD79 molecule, comprising two polypeptide chains, mb-1 (CD79a) and B29 (CD79b), is physically associated in the B-cell membrane with immunoglobulin [PMID: 7632952]. CD79a has been reported to exhibit a high degree of linage-specificity for B-cell differentiation, with a specificity of 88% and a sensitivity of 100% [PMID: 34672246].
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Immunofluorescence
IF shows positive staining in paraffin-embedded human colon. Anti-CD79a/MB-1 (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human skeletal muscle. Anti-CD79a/MB-1 (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
